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lkb laser densitometer  (Molecular Dynamics Inc)

 
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    Molecular Dynamics Inc lkb laser densitometer
    Lkb Laser Densitometer, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lkb+laser+densitometer/lkb+ultra+scan+xl+laser+densitometer/pmc06148257-71-30-50
    Average 90 stars, based on 1 article reviews
    lkb laser densitometer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitation Assay:

    Article Title: Differential Kinetics of Transcription Complex Assembly Distinguish Oocyte and Somatic 5S RNA Genes of Xenopus
    Article Snippet: .. For quantitation of the relative transcription efficiencies of two 5S gene templates in the same reactions, autoradiograms (taken within the linear range of the X-ray film) were scanned with an LKB laser densitometer or dried gels were analyzed by storage phosphorimage autoradiography and data analysis was performed using Image-Quant software (Molecular Dynamics). ..

    Autoradiography:

    Article Title: Differential Kinetics of Transcription Complex Assembly Distinguish Oocyte and Somatic 5S RNA Genes of Xenopus
    Article Snippet: .. For quantitation of the relative transcription efficiencies of two 5S gene templates in the same reactions, autoradiograms (taken within the linear range of the X-ray film) were scanned with an LKB laser densitometer or dried gels were analyzed by storage phosphorimage autoradiography and data analysis was performed using Image-Quant software (Molecular Dynamics). ..

    Software:

    Article Title: Differential Kinetics of Transcription Complex Assembly Distinguish Oocyte and Somatic 5S RNA Genes of Xenopus
    Article Snippet: .. For quantitation of the relative transcription efficiencies of two 5S gene templates in the same reactions, autoradiograms (taken within the linear range of the X-ray film) were scanned with an LKB laser densitometer or dried gels were analyzed by storage phosphorimage autoradiography and data analysis was performed using Image-Quant software (Molecular Dynamics). ..



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    Maternal uniparental disomy in a PWS patient with apparently normal chromosomes 15. a, Two pairs of intact chromosomes 15 of PWS2. Previous reports have indicated these chromosomes as being intact’3 or deleted 15q (ref. 14); in the deletion report a polymorphic shortening of 15q11 (ref. 7) was probably detected, because we show here at a higher level of resolution that the chromosomes are intact. b, Maternal origin of the two chromosomes 15 in PWS2. DNA was digested with SeaI. PWS2 (lane 1) inherits a maternal 6.3-kb allele (lane 3) but no paternal 6.5-kb allele (lane 2) for probe 34 (D15S9). Each individual is heterozygous for IR10-1 (D15S12; 16- and 12.5-kb alleles), which is from the same 15q11q13 region as probe 34 (ref. 10). The band at 10 kb is constant. C. for probe 34 in all individuals5 c. PWS2 inherits maternal alleles only for probe IR39d (D15S18). DNA was digested with SacI and hybridized with the 15q11q13-specific probe IR39d. PWS2 (lane 1) inherits his mother’s (lane 3) 14-kb alleles and not his father’s (lane 2) 8.5-kb alleles. d, Two copies of the maternal allele for probe 34 in PWS2. HindIII-digested DNAs from PWS2 (lane 2), mother M2 (lane 3), father F2 (lane 4) and a PWS patient displaying both a cytological and molecular deletion of 15q11q13 (ref. 5; lane 1. HS2) were hybridized simultaneously with probes 34 and H2-26 (D13S28)31 . Probe H2-26 from chromosome 13 serves as a reference for quantitation of copy number10. Probe 34 shows a reduced hybridization intensity relative to H2-26 only in HS2, which is consistent with a deletion of 15q11q13 in this individual. No reduction in probe 34 hybridization intensity is observed in PWS2 or his parents. To quantitate the intensity of the hybridization bands, the autoradiogram was scanned with an <t>LKB</t> <t>Ultrascan</t> XL laser <t>densitometer</t> (Pharmacia LKB Biotechnology). The area ratio of the probe 34 to probe H2-26 peaks was calculated for each lane. The number of copies of probe 34 per genome was obtained by setting the ratio in lane 3 (M2, mother) to 2 and normalizing the values in the other lanes to this value. The number of copies of probe 34 per genome determined by this method were 1.0 in lane 1 (the HS2 deletion), 1.8 in lane 2 (PWS2) and 2.2 in lane 4 (F2, father). e, Exclusion of non-paternity for PWS2. One maternal 5.2-kb (lane 2) and one paternal 2.9-kb (lane 3) PvuII allele are inherited by PWS2 (lane 1) for the 3’HVR. METHODS. DNA and cytogenetic studies were as for Fig. 1. Probe IR39d is a subclone of IR39 (ref. 10), which lacks repetitive sequences. PWS2 has been described before as patient number 5 (ref. 14) and patient number 31 (ref. 13).
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    Maternal uniparental disomy in a PWS patient with apparently normal chromosomes 15. a, Two pairs of intact chromosomes 15 of PWS2. Previous reports have indicated these chromosomes as being intact’3 or deleted 15q (ref. 14); in the deletion report a polymorphic shortening of 15q11 (ref. 7) was probably detected, because we show here at a higher level of resolution that the chromosomes are intact. b, Maternal origin of the two chromosomes 15 in PWS2. DNA was digested with SeaI. PWS2 (lane 1) inherits a maternal 6.3-kb allele (lane 3) but no paternal 6.5-kb allele (lane 2) for probe 34 (D15S9). Each individual is heterozygous for IR10-1 (D15S12; 16- and 12.5-kb alleles), which is from the same 15q11q13 region as probe 34 (ref. 10). The band at 10 kb is constant. C. for probe 34 in all individuals5 c. PWS2 inherits maternal alleles only for probe IR39d (D15S18). DNA was digested with SacI and hybridized with the 15q11q13-specific probe IR39d. PWS2 (lane 1) inherits his mother’s (lane 3) 14-kb alleles and not his father’s (lane 2) 8.5-kb alleles. d, Two copies of the maternal allele for probe 34 in PWS2. HindIII-digested DNAs from PWS2 (lane 2), mother M2 (lane 3), father F2 (lane 4) and a PWS patient displaying both a cytological and molecular deletion of 15q11q13 (ref. 5; lane 1. HS2) were hybridized simultaneously with probes 34 and H2-26 (D13S28)31 . Probe H2-26 from chromosome 13 serves as a reference for quantitation of copy number10. Probe 34 shows a reduced hybridization intensity relative to H2-26 only in HS2, which is consistent with a deletion of 15q11q13 in this individual. No reduction in probe 34 hybridization intensity is observed in PWS2 or his parents. To quantitate the intensity of the hybridization bands, the autoradiogram was scanned with an <t>LKB</t> <t>Ultrascan</t> XL laser <t>densitometer</t> (Pharmacia LKB Biotechnology). The area ratio of the probe 34 to probe H2-26 peaks was calculated for each lane. The number of copies of probe 34 per genome was obtained by setting the ratio in lane 3 (M2, mother) to 2 and normalizing the values in the other lanes to this value. The number of copies of probe 34 per genome determined by this method were 1.0 in lane 1 (the HS2 deletion), 1.8 in lane 2 (PWS2) and 2.2 in lane 4 (F2, father). e, Exclusion of non-paternity for PWS2. One maternal 5.2-kb (lane 2) and one paternal 2.9-kb (lane 3) PvuII allele are inherited by PWS2 (lane 1) for the 3’HVR. METHODS. DNA and cytogenetic studies were as for Fig. 1. Probe IR39d is a subclone of IR39 (ref. 10), which lacks repetitive sequences. PWS2 has been described before as patient number 5 (ref. 14) and patient number 31 (ref. 13).
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    Maternal uniparental disomy in a PWS patient with apparently normal chromosomes 15. a, Two pairs of intact chromosomes 15 of PWS2. Previous reports have indicated these chromosomes as being intact’3 or deleted 15q (ref. 14); in the deletion report a polymorphic shortening of 15q11 (ref. 7) was probably detected, because we show here at a higher level of resolution that the chromosomes are intact. b, Maternal origin of the two chromosomes 15 in PWS2. DNA was digested with SeaI. PWS2 (lane 1) inherits a maternal 6.3-kb allele (lane 3) but no paternal 6.5-kb allele (lane 2) for probe 34 (D15S9). Each individual is heterozygous for IR10-1 (D15S12; 16- and 12.5-kb alleles), which is from the same 15q11q13 region as probe 34 (ref. 10). The band at 10 kb is constant. C. for probe 34 in all individuals5 c. PWS2 inherits maternal alleles only for probe IR39d (D15S18). DNA was digested with SacI and hybridized with the 15q11q13-specific probe IR39d. PWS2 (lane 1) inherits his mother’s (lane 3) 14-kb alleles and not his father’s (lane 2) 8.5-kb alleles. d, Two copies of the maternal allele for probe 34 in PWS2. HindIII-digested DNAs from PWS2 (lane 2), mother M2 (lane 3), father F2 (lane 4) and a PWS patient displaying both a cytological and molecular deletion of 15q11q13 (ref. 5; lane 1. HS2) were hybridized simultaneously with probes 34 and H2-26 (D13S28)31 . Probe H2-26 from chromosome 13 serves as a reference for quantitation of copy number10. Probe 34 shows a reduced hybridization intensity relative to H2-26 only in HS2, which is consistent with a deletion of 15q11q13 in this individual. No reduction in probe 34 hybridization intensity is observed in PWS2 or his parents. To quantitate the intensity of the hybridization bands, the autoradiogram was scanned with an LKB Ultrascan XL laser densitometer (Pharmacia LKB Biotechnology). The area ratio of the probe 34 to probe H2-26 peaks was calculated for each lane. The number of copies of probe 34 per genome was obtained by setting the ratio in lane 3 (M2, mother) to 2 and normalizing the values in the other lanes to this value. The number of copies of probe 34 per genome determined by this method were 1.0 in lane 1 (the HS2 deletion), 1.8 in lane 2 (PWS2) and 2.2 in lane 4 (F2, father). e, Exclusion of non-paternity for PWS2. One maternal 5.2-kb (lane 2) and one paternal 2.9-kb (lane 3) PvuII allele are inherited by PWS2 (lane 1) for the 3’HVR. METHODS. DNA and cytogenetic studies were as for Fig. 1. Probe IR39d is a subclone of IR39 (ref. 10), which lacks repetitive sequences. PWS2 has been described before as patient number 5 (ref. 14) and patient number 31 (ref. 13).

    Journal: Nature

    Article Title: Genetic imprinting suggested by maternal heterodisomy in non-deletion Prader-Willi syndrome

    doi: 10.1038/342281a0

    Figure Lengend Snippet: Maternal uniparental disomy in a PWS patient with apparently normal chromosomes 15. a, Two pairs of intact chromosomes 15 of PWS2. Previous reports have indicated these chromosomes as being intact’3 or deleted 15q (ref. 14); in the deletion report a polymorphic shortening of 15q11 (ref. 7) was probably detected, because we show here at a higher level of resolution that the chromosomes are intact. b, Maternal origin of the two chromosomes 15 in PWS2. DNA was digested with SeaI. PWS2 (lane 1) inherits a maternal 6.3-kb allele (lane 3) but no paternal 6.5-kb allele (lane 2) for probe 34 (D15S9). Each individual is heterozygous for IR10-1 (D15S12; 16- and 12.5-kb alleles), which is from the same 15q11q13 region as probe 34 (ref. 10). The band at 10 kb is constant. C. for probe 34 in all individuals5 c. PWS2 inherits maternal alleles only for probe IR39d (D15S18). DNA was digested with SacI and hybridized with the 15q11q13-specific probe IR39d. PWS2 (lane 1) inherits his mother’s (lane 3) 14-kb alleles and not his father’s (lane 2) 8.5-kb alleles. d, Two copies of the maternal allele for probe 34 in PWS2. HindIII-digested DNAs from PWS2 (lane 2), mother M2 (lane 3), father F2 (lane 4) and a PWS patient displaying both a cytological and molecular deletion of 15q11q13 (ref. 5; lane 1. HS2) were hybridized simultaneously with probes 34 and H2-26 (D13S28)31 . Probe H2-26 from chromosome 13 serves as a reference for quantitation of copy number10. Probe 34 shows a reduced hybridization intensity relative to H2-26 only in HS2, which is consistent with a deletion of 15q11q13 in this individual. No reduction in probe 34 hybridization intensity is observed in PWS2 or his parents. To quantitate the intensity of the hybridization bands, the autoradiogram was scanned with an LKB Ultrascan XL laser densitometer (Pharmacia LKB Biotechnology). The area ratio of the probe 34 to probe H2-26 peaks was calculated for each lane. The number of copies of probe 34 per genome was obtained by setting the ratio in lane 3 (M2, mother) to 2 and normalizing the values in the other lanes to this value. The number of copies of probe 34 per genome determined by this method were 1.0 in lane 1 (the HS2 deletion), 1.8 in lane 2 (PWS2) and 2.2 in lane 4 (F2, father). e, Exclusion of non-paternity for PWS2. One maternal 5.2-kb (lane 2) and one paternal 2.9-kb (lane 3) PvuII allele are inherited by PWS2 (lane 1) for the 3’HVR. METHODS. DNA and cytogenetic studies were as for Fig. 1. Probe IR39d is a subclone of IR39 (ref. 10), which lacks repetitive sequences. PWS2 has been described before as patient number 5 (ref. 14) and patient number 31 (ref. 13).

    Article Snippet: To quantitate the intensity of the hybridization bands, the autoradiogram was scanned with an LKB Ultrascan XL laser densitometer (Pharmacia LKB Biotechnology).

    Techniques: Quantitation Assay, Hybridization